rabbit monoclonal antibody against il 1β  (Bioss)

Journal: PLoS Pathogens

Article Title: STING regulates metabolic reprogramming in macrophages via HIF-1α during Brucella infection

doi: 10.1371/journal.ppat.1009597

Figure Lengend Snippet: IL-1β (A) , TNF-α (B) and IL-10 (D) produced by macrophages derived from HIF-1α WT and HIF-1α KO mice, non-infected (NI) or infected with B . abortus , detected in cell supernatants using ELISA. (C) NO 2 − (nitrite) accumulation in the media of macrophages derived from HIF-1α WT and HIF-1α KO mice, non-infected (NI) or infected with B . abortus , measured by Griess reaction. (E) Western blot analysis of HIF-1α, pro-IL-1β, pro-caspase-1, caspase-11 and p30 fragment of GSDMD in cell lysates and caspase-1 (p20 subunit) in supernatants from macrophages derived from HIF-1α WT and HIF-1α KO mice, non-infected (NI) or infected with B . abortus (Ba). Equal loading was controlled by measuring β-actin in the corresponding cell lysates. (F) Cell death was determined by measuring the percentage of LDH release by B . abortus -infected macrophages derived from HIF-1α WT and HIF-1α KO mice. (G) IL-1β expression levels determined by real-time RT-PCR in B . abortus -infected macrophages derived from HIF-1α WT and HIF-1α KO mice. IL-1β released (H) and TNF-α secreted (I) from macrophages derived from C57BL/6 mice, non-infected (NI) or infected with B . abortus , and non-treated (vehicle) or pretreated with 1 mM 2-DG, detected in cell supernatants using ELISA. The data (A-I) are representative of three independent experiments. The data (A-D and H-I) are presented as mean ± SD, *p < 0.05, two-way ANOVA. The data (F-G) are presented as mean ± SD, *p < 0.05, Student’s t test.

Article Snippet: Primary antibodies used included a monoclonal against HIF-1α (clone D1S7W, Cell Signaling Technology, Danvers, MA), a monoclonal antibody against IL-1β (clone 3A6, Cell Signaling Technology), a monoclonal antibody against gasdermin D (clone GN20-13, Genentech, South San Francisco, CA), a monoclonal antibody against caspase-11 (clone Flamy-1, Adipogen, San Diego, CA), and a monoclonal antibody against the p20 subunit of caspase-1 (clone Casper-1, Adipogen), all at a 1:1000 dilution.

Techniques: Produced, Derivative Assay, Infection, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Quantitative RT-PCR

Journal: PLoS Pathogens

Article Title: STING regulates metabolic reprogramming in macrophages via HIF-1α during Brucella infection

doi: 10.1371/journal.ppat.1009597

Figure Lengend Snippet: (A) Western blot analysis of HIF-1α in cell lysates from macrophages derived from VHL WT and VHL KO mice, non-infected (NI) or infected with B . abortus (Ba). Equal loading was controlled by measuring β-actin in the corresponding cell lysates. (B) CCR7, NOS2, ARG1, and YM1 expression levels determined by real-time RT-PCR in macrophages derived from VHL WT and VHL KO mice infected with B . abortus . (C) GLUT1 expression levels determined by real-time RT-PCR in macrophages derived from VHL WT and VHL KO mice infected with B . abortus . IL-1β released (D) and TNF-α secreted (E) from macrophages derived from VHL WT and VHL KO mice, non-infected (NI) or infected with B . abortus , detected in cell supernatants using ELISA. (F) NO 2 − (nitrite) accumulation in the media of macrophages derived from VHL WT and VHL KO mice, non-infected (NI) or infected with B . abortus , measured by Griess reaction. The data (A-F) are representative of three independent experiments. The data (B and C) are presented as mean ± SD, *p < 0.05, Student’s t test. The data (D-F) are presented as mean ± SD, *p < 0.05, two-way ANOVA.

Article Snippet: Primary antibodies used included a monoclonal against HIF-1α (clone D1S7W, Cell Signaling Technology, Danvers, MA), a monoclonal antibody against IL-1β (clone 3A6, Cell Signaling Technology), a monoclonal antibody against gasdermin D (clone GN20-13, Genentech, South San Francisco, CA), a monoclonal antibody against caspase-11 (clone Flamy-1, Adipogen, San Diego, CA), and a monoclonal antibody against the p20 subunit of caspase-1 (clone Casper-1, Adipogen), all at a 1:1000 dilution.

Techniques: Western Blot, Derivative Assay, Infection, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: PLoS Pathogens

Article Title: STING regulates metabolic reprogramming in macrophages via HIF-1α during Brucella infection

doi: 10.1371/journal.ppat.1009597

Figure Lengend Snippet: Measurements of intracellular succinate levels by (A) liquid chromatography-mass spectrometry and by (B) Succinate Colorimetric Assay Kit in B . abortus -infected macrophages derived from C57BL/6 (WT) and STING KO mice. IL-1β (C) , TNF-α (D) and IL-10 (F) produced by macrophages derived from HIF-1α WT and HIF-1α KO mice, pretreated or not with succinate (5 mM) and then non-infected (NI) or infected with B . abortus , detected in cell supernatants using ELISA. (E) NO 2 − (nitrite) accumulation in the media of macrophages derived from HIF-1α WT and HIF-1α KO mice, pretreated or not with succinate (5 mM) and then non-infected (NI) or infected with B . abortus , measured by Griess reaction. (G) Western blot analysis of HIF-1α and pro-IL-1β in cell lysates and caspase-1 (p20 subunit) in supernatants from macrophages derived from C57BL/6 (WT) mice, pretreated or not with succinate (5 mM) and then non-infected (NI) or infected with B . abortus . Equal loading was controlled by measuring β-actin in the corresponding cell lysates. (H) IL-1β expression levels determined by real-time RT-PCR in B . abortus -infected macrophages derived from C57BL/6 (WT) mice, non-treated (NT) or pretreated with succinate (5 mM). (I) Western blot analysis of HIF-1α in cell lysates from C57BL/6 (WT) and STING KO mice, pretreated or not with succinate (5 mM) and then non-infected (NI) or infected with B . abortus (Ba). Equal loading was controlled by measuring β-actin in the corresponding cell lysates. The data (A-I) are representative of three independent experiments. The data (A and B) are presented as mean ± SD, & (comparison between WT and KO) or * [comparison between non-infected (NI, set to 100%) and infected], p < 0.05, one-way ANOVA. The data (C-F) are presented as mean ± SD, * (comparison between WT and KO) or & (comparison between non-treated and succinate-treated), p < 0.05, two-way ANOVA. The data (H) is presented as mean ± SD, *p < 0.05, Student’s t test.

Article Snippet: Primary antibodies used included a monoclonal against HIF-1α (clone D1S7W, Cell Signaling Technology, Danvers, MA), a monoclonal antibody against IL-1β (clone 3A6, Cell Signaling Technology), a monoclonal antibody against gasdermin D (clone GN20-13, Genentech, South San Francisco, CA), a monoclonal antibody against caspase-11 (clone Flamy-1, Adipogen, San Diego, CA), and a monoclonal antibody against the p20 subunit of caspase-1 (clone Casper-1, Adipogen), all at a 1:1000 dilution.

Techniques: Liquid Chromatography, Mass Spectrometry, Colorimetric Assay, Infection, Derivative Assay, Produced, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Quantitative RT-PCR, Comparison

Journal: World Journal of Gastroenterology : WJG

Article Title: A preliminary study of neck-stomach syndrome

doi: 10.3748/wjg.v13.i18.2575

Figure Lengend Snippet: Table 3

Article Snippet: Blots were blocked with 50 g/L non-fat dry milk in Tris-buffered saline (TBS) for 1 h at room temperature and then the membranes were incubated with 1:200 diluted monoclonal rabbit anti-rat antibodies against IL-1β (Sigma-Aldrich) or caspase-3 (Sigma-Aldrich) overnight at 4°C.

Techniques: